Analytical Profiles of Drug Substances, Vol. 13 by Klaus Florey (Ed.)

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By Klaus Florey (Ed.)

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1 . . 1 . . 1 . . 1 . . 1 . . 1 ' . . 110 , * 8B 70 60 Fig. 4. 5 0 fPM(b) 44 44 20 PMR spectrum of camphor in CDCl 3' 0 40 J A B E R S . M O S S A A N D M A H M O U D M . A . HASSAN Table 4. PMR C h a r a c t e r i s t i c s o f Camphor Group Methylene a t ( C-2, Chemical s h i f t (ppm) C-4 and C-5 ) . 43 b t and Methine a t C-3 p r o t o n s . 83 s C-10 M e s = singlet ; t = t r i p l e t ; b t = b r o a d t r i p l e t ; rn = m u l t i p l e t . Our assignment o f t h e methyl p r o t o n s s i n g l e t s i s b a s e d on t h e a n i s o t r o p y of t h e c a r b o n y l f u n c t i o n o f camphor.

Pathol. Pharmacol. I + 31 (1976). 14. Ha. J. 11. Medico-ScientiTorino, 1978. G. D. Johnston, J. K e l l y , J. G. G. Shan Shanks, Clin. Pharmacol. Ther. 20 524 (1976). 16. D. F i t z g e r a l d , R. R u f f i n , I<. GTimedsted, R. Roberts, J. McAinsh, Europ. J. Clin. Pharmacol. 81 (1978). Ma Kaye, B r i t . J. Clin. Pharmacol. C. G. i(1974) . NcLean, 3. G. Seinzow, %ugs (suppl. 21, 131 (1983). J. D. F i t z g e r a l d , J. J. T. Simpson, B r i t . J. Clin. Pharmacol. 2, 267 (1976). 20. H. Z.

3 . Hiah Pressure Liquid The apparatus used for high pressure liquid chromatography was by Fye-Unicam (Cambridge), LPU-4011 equipped with a PU-4020 UV detector. 6 mm ID, 6 mm OD). The mobile phase was acetonitrile-dist. 5. In some analyses benzimidazole was used as an inOernal standard. 0 ml/min. S. 05 M in NaC1 were used. 4. Determination of Impurities s The "dimeric" com3ound , which may be present i n atenolol can be detec ed by all chromatographic methods and determined by Sas-liquid and I3PL chromatography.

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